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Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations in biological samples, but techniques for recovering three-dimensional orientations and positions of fluorescent ensembles are limited. We report a polarized dual-view light-sheet system for determining the diffraction-limited three-dimensional distribution of the orientations and positions of ensembles of fluorescent dipoles that label biological structures. We share a set of visualization, histogram, and profiling tools for interpreting these positions and orientations. We model the distributions based on the polarization-dependent efficiency of excitation and detection of emitted fluorescence, using coarse-grained representations we call orientation distribution functions (ODFs). We apply ODFs to create physics-informed models of image formation with spatio-angular point-spread and transfer functions. We use theory and experiment to conclude that light-sheet tilting is a necessary part of our design for recovering all three-dimensional orientations. We use our system to extend known two-dimensional results to three dimensions in FM1-43-labeled giant unilamellar vesicles, fast-scarlet-labeled cellulose in xylem cells, and phalloidin-labeled actin in U2OS cells. Additionally, we observe phalloidin-labeled actin in mouse fibroblasts grown on grids of labeled nanowires and identify correlations between local actin alignment and global cell-scale orientation, indicating cellular coordination across length scales. 

We study both theoretically and experimentally the effect of nonlinearity on topologically protected linear interface modes in a photonic Su–Schrieffer–Heeger (SSH) lattice. It is shown that under either focusing or defocusing nonlinearity, this linear topological mode of the SSH lattice turns into a family of topological gap solitons. These solitons are stable. However, they exhibit only a low amplitude and power and are thus weakly nonlinear, even when the bandgap of the SSH lattice is wide. As a consequence, if the initial beam has modest or high power, it will either delocalize, or evolve into a soliton not belonging to the family of topological gap solitons. These theoretical predictions are observed in our experiments with optically induced SSH-type photorefractive lattices.